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KMID : 0377220020270020009
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Medical Journal of Chosun Univercity
2002 Volume.27 No. 2 p.9 ~ p.27
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Immunocytochemical Study on the Parvalbumin and Calbindin D-28K Positive Neurons in the Rat Spinal Cord after Transection
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Kim Jong-Joong
Park Young-Lan
Moon Jeong-Seok
Chung Yoon-Young
Im You-Tack
Park Sang-Soo
Kim Hyun-Kon
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Abstract
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Background and Objectives: This study was done to compared the distribution of the two calcium-binding proteins immunoreactive neurons, parvalbumin immunoreactiv (PV-IR) and calbindin D-28K immunoreactive (CB-IR) neurons in the spinal cord after transection.
Materials and Methods: Twenty rats were divided into 3 groups (control, complete spinal cord injury (SCI), and right and left spinal cord hemisection). SCI was produced by cutting the spinal cord use blades 11 with scalpel handles.
Results: In this experiment, CB-IR neurons were mainly found in many pyramidal cells distributed in the brain stem and spinal cord of rats. PV-IR Neurons were demonstrated in all lamina of the gray matter of the spinal cord. These immunoreactive cells had the highest density in the layer I and II of dorsal horn and several nuclei of the ventral horn of the all the segments of the spinal cord. CB-IR neuropil labeling was strongly noted in all the segments of the spinal cord. In contrast PV-IR neurons were different in distribution, size and morphology in the spinal cord. The number of PV-IR neurons were greater than in the spinal cord compared with the CB-IR neurons. CB-IR and PV-IR somata were round, oval, spindle and polygonal in shape, and were unipolar, bipolar, multipolar and horizontal in types. The diameters of the somata of the PV-IR and CB-IR neurons were 40-50 ㎛, respectively. Also dendrites of PV-IR and CB-IR neurons were densely arrayed in network.
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KEYWORD
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Spinal cord injury, Calbindin D-28K, Parvalbumin
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